Lipophilic Tracer DiI Used for Neuronal Tracing in the Fixed Hippocampal Formations of Mice

Both the hippocampus and entorhinal cortex are involved in memory formation. It is thought that they work together to create a loop formation of long term memories. The sends projections CA1 field back cortex. How exactly communicate unknown. In order do electrophysiological studies it necessary know which orientation cut brain preserve most connections between these two areas. Here we show DiI crystal application can be used successfully label known fiber paths from dentate gyrus CA3 field. Then begin with sagittal horizontal slices see how well EC preserved.